LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast

Yoon-Dong Park; Won-Hwa Kang; Wan-Soo Yang; Kwang-Soo Shin; Kyung Sook Bae; Hee-Moon Park

doi:10.1016/j.bbrc.2003.10.110

LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast

Biochem Biophys Res Commun. 2003 Nov 28;311(4):1078-83. doi: 10.1016/j.bbrc.2003.10.110.

Authors

Yoon-Dong Park¹, Won-Hwa Kang, Wan-Soo Yang, Kwang-Soo Shin, Kyung Sook Bae, Hee-Moon Park

Affiliation

¹ Department of Microbiology, Chungnam National University, 305-764, Daejeon, Republic of Korea.

PMID: 14623292
DOI: 10.1016/j.bbrc.2003.10.110

Abstract

Previously, we reported that the LAMMER kinase homolog, Lkh1, is a negative regulator of filamentous growth and asexual flocculation in the fission yeast, Schizosaccharomyces pombe. Here, we report that the lkh1(+) null mutant is sensitive to oxidative stress because of a reduction in the expression of genes for antioxidant enzymes such as catalase (ctt1(+)) and Cu,Zn-superoxide dismutase (sod1(+)). Furthermore, the lkh1(+) null mutant shows increased levels of intracellular peroxides under conditions of oxidative stress compared with wild-type cells. Interestingly, expression of the gene for the transcription factor Atf1 is reduced in the lkh1(+) null mutant under oxidative stress, whereas expression of the transcription factor Pap1 is not. We report the novel finding that Lkh1 is involved in the oxidative-stress response of the fission yeast, S. pombe, and regulates the expression of antioxidant enzymes via the transcription factor Atf1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Activating Transcription Factor 1
Catalase / genetics
Catalase / metabolism*
Cell Survival / drug effects
Cell Survival / physiology
DNA-Binding Proteins*
Gene Expression Regulation, Enzymologic / physiology
Gene Expression Regulation, Fungal / physiology
Hydrogen Peroxide / pharmacology
Oxidative Stress / physiology*
Pancreatitis-Associated Proteins
Protein Kinases / genetics*
Protein Kinases / metabolism*
Schizosaccharomyces / drug effects
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism*
Superoxide Dismutase / genetics
Superoxide Dismutase / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism*

Substances

Activating Transcription Factor 1
DNA-Binding Proteins
Pancreatitis-Associated Proteins
REG3A protein, human
Schizosaccharomyces pombe Proteins
Transcription Factors
Hydrogen Peroxide
Catalase
Superoxide Dismutase
Protein Kinases
lkh1 protein, S pombe