Objective: To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China.
Methods: cDNA of Der f 2 was cloned by RT-PCR, screened and their sequences were analyzed.
Results: cDNA of Der f 2 was cloned. The sequence of the cloned Der f 2 was different with that published (D10448) in GenBank, with 87 additional nucleotides inserted into the 62th nucleotide of the original one. According to the original ORF, the deduced amino acids that were located prior or after the inserted 29 amino acid sequence showed no changes.
Conclusion: The cDNA of Der f 2 was cloned from Dermatophagoides farinae and its sequence showed significant difference with that reported in the GenBank.