Assignment of human sprouty 4 gene to chromosome segment 5q32 approximately 33 and analysis of its pattern of expression

J Genet. 2003 Apr-Aug;82(1-2):23-6. doi: 10.1007/BF02715877.

Abstract

The human sprouty 4 (SPRY4) gene was localized to chromosome band 5q32 approximately 33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPRY4 was expressed in all tested tissues to different levels.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 5*
  • DNA Primers / chemistry
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / metabolism
  • Radiation Hybrid Mapping
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution

Substances

  • DNA Primers
  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Proteins
  • RNA, Messenger
  • SPRY4 protein, human