CD8+-T-cell responses of Mycobacterium-infected mice to a newly identified major histocompatibility complex class I-restricted epitope shared by proteins of the ESAT-6 family

Laleh Majlessi; Marie-Jésus Rojas; Priscille Brodin; Claude Leclerc

doi:10.1128/IAI.71.12.7173-7177.2003

CD8+-T-cell responses of Mycobacterium-infected mice to a newly identified major histocompatibility complex class I-restricted epitope shared by proteins of the ESAT-6 family

Infect Immun. 2003 Dec;71(12):7173-7. doi: 10.1128/IAI.71.12.7173-7177.2003.

Authors

Laleh Majlessi¹, Marie-Jésus Rojas, Priscille Brodin, Claude Leclerc

Affiliation

¹ Unité de Biologie des Régulations Immunitaires, INSERM E352, Institut Pasteur, Paris, France. [email protected]

Abstract

Here we describe the identification of a new CD8(+)-T-cell epitope, the GYAGTLQSL nonamer, shared by the TB10.3 and TB10.4 proteins of the Mycobacterium tuberculosis ESAT-6 family. Cytotoxic T cells from mycobacterium-infected mice efficiently recognized this epitope. GYAGTLQSL-specific T-cell hybridomas, which were able to recognize Mycobacterium bovis BCG-infected macrophages, were generated and now allow investigation of mycobacterial-antigen processing through the major histocompatibility complex class I pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antigens, Bacterial / immunology*
Bacterial Proteins
CD8-Positive T-Lymphocytes / immunology*
Epitopes, T-Lymphocyte / chemistry
Epitopes, T-Lymphocyte / immunology*
Histocompatibility Antigens Class I / metabolism*
Hybridomas
Immunization
Macrophages / microbiology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mycobacterium bovis / immunology
Mycobacterium tuberculosis / immunology
T-Lymphocytes, Cytotoxic / immunology
Tuberculosis, Pulmonary / immunology*
Tuberculosis, Pulmonary / microbiology

Substances

Antigens, Bacterial
Bacterial Proteins
ESAT-6 protein, Mycobacterium tuberculosis
Epitopes, T-Lymphocyte
Histocompatibility Antigens Class I