Abstract
The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.
Copyright 2003 Wiley-Liss, Inc.
MeSH terms
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Animals
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Biomarkers, Tumor / metabolism
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Cell Movement / drug effects
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Cell Movement / physiology*
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology
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Colonic Neoplasms / prevention & control*
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Enzyme Activation
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Epidermal Growth Factor / pharmacology
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Humans
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In Vitro Techniques
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Liver Neoplasms / metabolism
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Liver Neoplasms / prevention & control*
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Liver Neoplasms / secondary*
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Mice
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Mice, Nude
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Monomeric GTP-Binding Proteins / genetics
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Monomeric GTP-Binding Proteins / physiology*
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Myosin Light Chains / metabolism*
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NM23 Nucleoside Diphosphate Kinases
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Nucleoside-Diphosphate Kinase*
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Phosphorylation
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transfection
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Tumor Cells, Cultured
Substances
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Biomarkers, Tumor
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Myosin Light Chains
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NM23 Nucleoside Diphosphate Kinases
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Transcription Factors
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Epidermal Growth Factor
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Mitogen-Activated Protein Kinase Kinases
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NME1 protein, human
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Nme1 protein, mouse
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Nucleoside-Diphosphate Kinase
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Monomeric GTP-Binding Proteins