Abstract
Objective:
To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats.
Methods:
With carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed.
Results:
Decoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10.
Conclusions:
Decoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cells, Cultured
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Dose-Response Relationship, Drug
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Female
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Inflammation Mediators / metabolism*
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Interleukin-10 / metabolism
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Interleukin-6 / metabolism
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Male
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Molecular Sequence Data
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NF-kappa B / drug effects*
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NF-kappa B / metabolism*
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Oligodeoxyribonucleotides / pharmacology*
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RNA, Messenger / analysis
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Random Allocation
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Rats
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Rats, Wistar
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Reference Values
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sensitivity and Specificity
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Tumor Necrosis Factor-alpha / drug effects
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Inflammation Mediators
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Interleukin-6
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NF-kappa B
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Oligodeoxyribonucleotides
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Interleukin-10