Abstract
To determine if lysophosphatidylcholine (lysoPC) is able to induce proinflammatory changes in monocytes, its ability to stimulate arachidonic acid (AA) release, a product of phospholipase A2 (PLA(2)) activity, has been analyzed. LysoPC increased AA release in THP-1 and Mono Mac6 cells in a time- and concentration-dependent manner. The monocytes expressed both secretory and cytosolic PLA(2) enzymes and AA release was strongly reduced by cellular pretreatment with different PLA(2) inhibitors and by pertussis toxin, an inhibitor of G(i)-protein activation. This indicates that both cytosolic and secretory PLA(2) enzymes regulate specific lysoPC receptor-induced AA release, suggesting lysoPC participation in monocyte proinflammatory activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arachidonic Acid / chemistry
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Arachidonic Acid / metabolism*
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Arachidonic Acids / pharmacology
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Carbon Radioisotopes
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Cell Differentiation / physiology
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Cell Line
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Cytosol / enzymology
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Cytosol / metabolism
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Humans
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Lysophosphatidylcholines / pharmacology*
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Monocytes / drug effects
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Monocytes / metabolism*
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Oleic Acid / chemistry
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Oleic Acid / metabolism
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Organophosphonates / pharmacology
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Pertussis Toxin / pharmacology
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Phospholipases A / antagonists & inhibitors
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Phospholipases A / metabolism
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Phospholipases A / physiology*
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Phospholipases A2
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Time Factors
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Tritium
Substances
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Arachidonic Acids
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Carbon Radioisotopes
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Enzyme Inhibitors
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Lysophosphatidylcholines
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Organophosphonates
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methyl arachidonylfluorophosphonate
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Tritium
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Arachidonic Acid
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Oleic Acid
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Pertussis Toxin
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Phospholipases A
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Phospholipases A2