Abstract
Harpagophytum procumbens (Pedaliaceae) has been used for the treatment of pain and arthritis. The effect of Harpagophytum procumbens against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E(2) (PGE(2)) immunoassay, and nitric oxide detection on mouse fibroblast cell line L929. The aqueous extract of Harpagophytum procumbens was shown to suppress PGE(2) synthesis and nitric oxide production by inhibiting lipopolysaccharide-stimulated enhancement of the cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) mRNAs expressions in L929 cells. These results suggest that Harpagophytum procumbens exerts anti-inflammatory and analgesic effects probably by suppressing cyclooxygenase-2 and iNOS expressions.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cyclooxygenase 2
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Dose-Response Relationship, Drug
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Fibroblasts / drug effects*
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Fibroblasts / enzymology
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / physiology
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Harpagophytum*
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Isoenzymes / antagonists & inhibitors*
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Isoenzymes / biosynthesis
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Lipopolysaccharides / pharmacology
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Mice
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Nitric Oxide Synthase / antagonists & inhibitors*
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Nitric Oxide Synthase / biosynthesis
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Nitric Oxide Synthase Type II
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Prostaglandin-Endoperoxide Synthases / biosynthesis
Substances
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Isoenzymes
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Lipopolysaccharides
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Plant Extracts
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Cyclooxygenase 2
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Prostaglandin-Endoperoxide Synthases