Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau)

Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11809-13. doi: 10.1073/pnas.89.24.11809.

Abstract

Previous studies have indicated that human transcription initiation factor TFIID is a large complex that contains a TATA-binding polypeptide (TFIID tau or TBP) and other components that qualitatively alter promoter interactions and are uniquely required for activator-dependent (versus basal) transcription. TFIID tau-specific antibody columns have been employed to identify a number of human TFIID polypeptides that are tightly associated with TFIID tau. These differ in size from polypeptides in known general initiation factors, including the initiator-binding factor (TFII-I) which shares some promoter binding characteristics with TFIID. The largest component (p250) identified in TFIID was shown to interact directly and tightly with TFIID tau, suggesting that it may play a major role in the assembly of the TFIID complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA-Binding Proteins / chemistry*
  • Humans
  • Immunologic Techniques
  • Macromolecular Substances
  • Molecular Weight
  • Protein Binding
  • RNA Polymerase II / metabolism
  • TATA Box*
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors / chemistry*
  • Transcription Factors / immunology

Substances

  • DNA-Binding Proteins
  • Macromolecular Substances
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors
  • RNA Polymerase II