Abstract
A sucrose: sucrose 1-fructosyltransferase (1-SST) gene and cDNA (Lp 1-SST) from perennial ryegrass (Lolium perenne) were isolated. The Lp 1-SST gene was fully sequenced and shown to contain three exons and two introns. Nucleotide sequence analysis of the 4824 bp Lp 1-SST genomic sequence revealed 1618 bp of 5' UTR and an open reading frame of 1962 bp encoding a protein of 653 amino acids. Lp 1-SST is 95% identical to the tall fescue 1-SST and contains plant fructosyltransferase functional domains. Lp 1-SST corresponds to a single copy gene in perennial ryegrass, and is expressed in young leaf bases and mature leaf sheaths. The recombinant Lp 1-SST protein from corresponding cDNA expression in Pichia pastoris showed 1-SST activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Plant
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Hexosyltransferases / genetics*
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Hexosyltransferases / isolation & purification
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Hexosyltransferases / metabolism
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Lolium / enzymology*
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Lolium / genetics
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Molecular Sequence Data
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Phylogeny
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Plant Leaves / genetics
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Plant Leaves / metabolism
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Analysis, DNA
Substances
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DNA, Complementary
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Plant Proteins
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Recombinant Fusion Proteins
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Hexosyltransferases
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inulosucrase
Associated data
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GENBANK/AF481763
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GENBANK/AY082350
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GENBANK/AY245431