Abstract
Pactolus is a cell surface protein expressed by murine neutrophils. Pactolus is similar to the beta integrins, except it lacks a functional metal ion-dependent adhesion site domain and is expressed without an alpha-chain partner. The majority of the Pactolus protein is held within the cell in dense granules in a highly glycosylated form. This intracellular form of Pactolus can be released to the cell surface following inflammatory activation or ligation of Pactolus on the cell surface. In addition, intracellular Pactolus translocates to the neutrophil surface following induction of apoptosis. Neutrophil activation studies suggest that Pactolus does not serve as an activating or phagocytic receptor for the neutrophil. To further define the function of Pactolus, a Pactolus-null mouse was generated. Pactolus-deficient animals mature appropriately and possess normal numbers of neutrophils, display appropriate migration into sites of inflammation, and combat introduced infections efficiently. These data suggest that Pactolus does not function as a neutrophil phagocytic or adhesion receptor, but may instead serve as a sugar-bearing ligand for lectin recognition by other cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Apoptosis / genetics
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Apoptosis / immunology*
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Cell Movement / genetics
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Cell Movement / immunology*
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Exocytosis / genetics
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Exocytosis / immunology
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Genetic Predisposition to Disease
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Integrin beta1 / genetics
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Integrin beta1 / metabolism*
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Integrin beta1 / physiology
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Intracellular Fluid / immunology
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Intracellular Fluid / metabolism
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Leukotriene B4 / pharmacology
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Membrane Proteins / agonists
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Membrane Proteins / deficiency
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Neutrophil Activation / genetics
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Neutrophil Activation / immunology*
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Neutrophil Infiltration / genetics
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Neutrophil Infiltration / immunology*
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Neutrophils / cytology*
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Neutrophils / immunology
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Neutrophils / metabolism*
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Phagocytosis / genetics
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Phagocytosis / immunology
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Protein Transport / genetics
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Protein Transport / immunology
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Signal Transduction / drug effects
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Signal Transduction / genetics
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Signal Transduction / immunology
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Staphylococcal Infections / genetics
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Staphylococcal Infections / immunology
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Staurosporine / pharmacology
Substances
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Integrin beta1
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Membrane Proteins
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pactolus
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Leukotriene B4
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Staurosporine