[Identification of Mycobacterium tuberculosis strains and a simultaneous identification of their drug resistance by the hybridization method on oligonucleotide microchips]

D A Griadunov; V M Mikhaĭlovich; S A Lapa; N I Rudinskiĭ; V E Barskiĭ; A V Chudinov; A S Zasedatelev; A D Mirzabekov

[Identification of Mycobacterium tuberculosis strains and a simultaneous identification of their drug resistance by the hybridization method on oligonucleotide microchips]

Mol Gen Mikrobiol Virusol. 2003:(4):24-7.

[Article in Russian]

Authors

D A Griadunov, V M Mikhaĭlovich, S A Lapa, N I Rudinskiĭ, V E Barskiĭ, A V Chudinov, A S Zasedatelev, A D Mirzabekov

PMID: 14664159

Abstract

A method of multiplex polymerase chain reaction (PCR) with subsequent hyoridization on oligonucleotide microchips was worked out to identify the Mycobacterium tuberculosis complex and to determine simultaneously the bacterial sensitivity to 2 first-line drugs, i.e. rifampin and isoniazid. The method provides for detecting above 95% of rifampin-resistant and around 80% of isoniazid-resistant strains within 1 day.

Publication types

English Abstract

MeSH terms

Bacterial Proteins*
Catalase*
Drug Resistance, Bacterial / genetics*
Humans
Isoniazid / pharmacology
Mutation
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Oligonucleotide Array Sequence Analysis / instrumentation
Oligonucleotide Array Sequence Analysis / methods*
Oligonucleotides / genetics
Oxidoreductases / genetics
Rifampin / pharmacology

Substances

Bacterial Proteins
Oligonucleotides
Oxidoreductases
Catalase
katG protein, Mycobacterium tuberculosis
Isoniazid
Rifampin