Abstract
A global map of gene expression within an organ can identify genes with coordinated expression in localized domains, thereby relating gene activity to cell fate and tissue specialization. Here, we present localization of expression of more than 22,000 genes in the Arabidopsis root. Gene expression was mapped to 15 different zones of the root that correspond to cell types and tissues at progressive developmental stages. Patterns of gene expression traverse traditional anatomical boundaries and show cassettes of hormonal response. Chromosomal clustering defined some coregulated genes. This expression map correlates groups of genes to specific cell fates and should serve to guide reverse genetics.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Arabidopsis / cytology
-
Arabidopsis / genetics*
-
Arabidopsis / growth & development
-
Arabidopsis / metabolism
-
Cell Separation
-
Chromosome Mapping
-
Chromosomes, Plant / genetics
-
Gene Expression Profiling*
-
Gene Expression Regulation, Plant
-
Gene Expression*
-
Genes, Plant
-
Green Fluorescent Proteins
-
Luminescent Proteins / analysis
-
Meristem / cytology
-
Meristem / genetics
-
Meristem / growth & development
-
Meristem / metabolism
-
Oligonucleotide Array Sequence Analysis
-
Plant Growth Regulators / physiology
-
Plant Root Cap / cytology
-
Plant Root Cap / genetics
-
Plant Root Cap / growth & development
-
Plant Root Cap / metabolism
-
Plant Roots / cytology
-
Plant Roots / genetics*
-
Plant Roots / growth & development
-
Plant Roots / metabolism
-
Protoplasts
-
RNA, Messenger / analysis
-
RNA, Messenger / genetics
-
RNA, Plant / analysis
-
RNA, Plant / genetics
-
Reverse Transcriptase Polymerase Chain Reaction
-
Signal Transduction / genetics
-
Transcription Factors / genetics
-
Transcription Factors / physiology
Substances
-
Luminescent Proteins
-
Plant Growth Regulators
-
RNA, Messenger
-
RNA, Plant
-
Transcription Factors
-
Green Fluorescent Proteins