Abstract
4-1BB Ligand (4-1BBL), a transmembrane molecule, member of the tumor necrosis factor ligand superfamily, is an important costimulatory molecule in the immune response. In this study a functional anti-human 4-1BBL MAb 1F1 was obtained and the specificity of this MAb was verified by flow cytometry and Western blotting. This MAb effectively recognized the 4-1BBL molecule expressed on a series of malignant cell lines as well as on DC and monocytes and it inhibited the proliferation of T lymphocytes, costimulated by soluble 4-1BBL and agonist anti-human CD3 MAb. Furthermore, we demonstrated that MAb 1F1 induced an impressive proliferation of monocytes from peripheral blood by triggering the reverse signal through 4-1BBL. This functional anti-human 4-1BBL MAb provides a valuable tool for further study of biological functions as well as signal transduction of 4-1BBL/4-1BB.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
4-1BB Ligand
-
Animals
-
Antibodies, Monoclonal / immunology
-
Antigens, CD
-
Cell Line
-
Humans
-
Immunoglobulin G / immunology
-
Mice
-
Mice, Inbred BALB C
-
Monocytes / immunology
-
Monocytes / metabolism
-
Receptors, Nerve Growth Factor / immunology*
-
Receptors, Nerve Growth Factor / metabolism
-
Receptors, Tumor Necrosis Factor / immunology*
-
Receptors, Tumor Necrosis Factor / metabolism
-
T-Lymphocytes / immunology
-
Tumor Cells, Cultured
-
Tumor Necrosis Factor Receptor Superfamily, Member 9
-
Tumor Necrosis Factor-alpha / immunology*
-
Tumor Necrosis Factor-alpha / metabolism
Substances
-
4-1BB Ligand
-
Antibodies, Monoclonal
-
Antigens, CD
-
Immunoglobulin G
-
Receptors, Nerve Growth Factor
-
Receptors, Tumor Necrosis Factor
-
TNFRSF9 protein, human
-
TNFSF9 protein, human
-
Tnfrsf9 protein, mouse
-
Tnfsf9 protein, mouse
-
Tumor Necrosis Factor Receptor Superfamily, Member 9
-
Tumor Necrosis Factor-alpha