Characterisation of Hungarian porcine circovirus 2 genomes associated with PMWS and PDNS cases

Acta Vet Hung. 2003;51(4):551-62. doi: 10.1556/AVet.51.2003.4.13.

Abstract

The authors report the data of the first survey on the incidence of post-weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS) in Hungary. A PCR method specific for the detection of porcine circovirus 2 (PCV-2) was developed, which proved to be suitable for diagnostic purposes. PCR screening of organ samples from pigs suspected to be affected with PMWS or PDNS revealed the presence of PCV-2 in 80% of the cases. Six PCV-2 genomes from Hungarian isolates were completely sequenced. Phylogenetic comparison with all the available PCV-2 sequences showed that porcine circoviruses circulating in Hungary are more variable than in several other European countries. Two Hungarian strains clustered together with the Spanish strains forming a distinct group; two others fell in a common group with the French, UK, and Dutch strains, whereas another two strains showed the closest relationship to two of the three known German PCV-2 sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circoviridae Infections / epidemiology
  • Circoviridae Infections / veterinary*
  • Circoviridae Infections / virology
  • Circovirus / genetics*
  • Circovirus / isolation & purification
  • DNA Primers
  • DNA, Viral / analysis
  • Genome
  • Hungary / epidemiology
  • Incidence
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / veterinary
  • Prevalence
  • Swine
  • Swine Diseases / epidemiology*
  • Swine Diseases / virology*
  • Wasting Syndrome / epidemiology
  • Wasting Syndrome / veterinary*
  • Wasting Syndrome / virology

Substances

  • DNA Primers
  • DNA, Viral

Associated data

  • GENBANK/AY256455
  • GENBANK/AY256456
  • GENBANK/AY256457
  • GENBANK/AY256458
  • GENBANK/AY256459
  • GENBANK/AY256460