Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative

Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):190-2. doi: 10.1107/s0907444903026222. Epub 2003 Dec 18.

Abstract

The pectate lyase PelI is involved in the degradation of plant tissues by the phytopathogenic bacterium Erwinia chrysanthemi. It has been crystallized from a solution containing PEG 550 in the space group P2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 A, beta = 112.8 degrees. Crystals diffract to 1.45 A using synchrotron radiation. SAD phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (L(III) edge).

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Dickeya chrysanthemi / enzymology*
  • Dickeya chrysanthemi / genetics
  • Escherichia coli / genetics
  • Gold / chemistry
  • Polysaccharide-Lyases / chemistry*
  • Polysaccharide-Lyases / genetics
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics

Substances

  • DNA, Bacterial
  • Recombinant Proteins
  • Gold
  • Polysaccharide-Lyases
  • pectate lyase