Immunological characterization of a 17-kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies

FEMS Microbiol Lett. 1992 Nov 15;78(1):7-14. doi: 10.1016/0378-1097(92)90280-2.

Abstract

Cryptosporidium parvum antigens were characterized by immunoblot analysis of sera and intestinal secretions of BALB/c mice orally infected with 10(5) oocysts. A major band at 17 kDa under non-reduced conditions and at 18 kDa under reduced conditions was recognized by anti-C. parvum IgA and IgG in serum and intestinal secretions from day 15 post-infection. This recognition persisted throughout the experiment (day 30). Mouse-serum antibodies raised against the 17-kDa purified antigen (P17) showed no cross-reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Protozoan / analysis
  • Antibodies, Protozoan / immunology*
  • Antigens, Protozoan / analysis
  • Antigens, Protozoan / immunology*
  • Cryptosporidiosis / immunology*
  • Cryptosporidium parvum / immunology*
  • Immunoglobulin A, Secretory / analysis
  • Immunoglobulin A, Secretory / immunology*
  • Immunoglobulin G / analysis
  • Immunoglobulin G / immunology
  • Immunoglobulin M / analysis
  • Immunoglobulin M / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Immunoglobulin A, Secretory
  • Immunoglobulin G
  • Immunoglobulin M