Multiharmonic-generation biopsy of skin

Opt Lett. 2003 Dec 15;28(24):2488-90. doi: 10.1364/ol.28.002488.

Abstract

Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200-1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biopsy / methods*
  • Dermis / pathology
  • Ear / pathology
  • Lasers
  • Mice
  • Photons
  • Skin / pathology*