Abstract
It has been shown previously that leukaemia inhibitory factor (LIF) and transforming growth factor-alpha (TGF-alpha) stimulate proliferation of primary cultures of murine myoblasts. We now show that human myoblasts respond in a similar manner to LIF and TGF-alpha. These responses occur over a range of growth conditions. There are total additive effects in both human and murine myoblasts between LIF and TGF-alpha and LIF and fibroblast growth factor-beta (FGF-beta), but not between LIF and interleukin-6 (IL-6) or insulin-like growth factor 1 (IGF-1). The LIF response is initiated by a short exposure to the cytokine and is maintained for prolonged periods in its absence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / pharmacology
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Cell Division / drug effects
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Cytokines / pharmacology*
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Growth Inhibitors / pharmacology*
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Humans
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Hybridomas / immunology
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Interleukin-6*
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Leukemia Inhibitory Factor
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Lymphokines / pharmacology*
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Mice
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Mice, Inbred C57BL
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Muscles / cytology*
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Muscles / drug effects
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Platelet-Derived Growth Factor / pharmacology
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Transforming Growth Factor alpha / pharmacology
Substances
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Antibodies, Monoclonal
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Cytokines
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Growth Inhibitors
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Interleukin-6
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LIF protein, human
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Leukemia Inhibitory Factor
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Lif protein, mouse
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Lymphokines
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Platelet-Derived Growth Factor
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Transforming Growth Factor alpha