Cloning, characterization and comparative analysis of pig plasma apolipoprotein A-IV

Gene. 2004 Jan 21:325:157-64. doi: 10.1016/j.gene.2003.10.007.

Abstract

Pig apolipoprotein (apo) A-IV cDNA was cloned, characterized and compared to the human ortholog. Mature porcine apo A-IV consists of 362 amino acids and displays a 75.6% sequence identity with human protein. Pig apo A-IV is the smallest reported mammalian apo A-IV because it lacks the repeated motifs of glutamine and glutamic acid at the carboxyl terminus. A phylogenic tree of apo A-IV mammalian proteins reveals that porcine apo A-IV is more closely related to humans and primates than to rodents. This protein is highly hydrophobic and is mainly associated with lipoproteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apolipoproteins A / blood
  • Apolipoproteins A / chemistry
  • Apolipoproteins A / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Hydrophobic and Hydrophilic Interactions
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Swine / genetics*

Substances

  • Apolipoproteins A
  • DNA, Complementary
  • apolipoprotein A-IV

Associated data

  • GENBANK/AJ222966