Immunolabelling of prolactin at ultrastructural level using the protein A-gold technique on Epon-embedded tissue

Malays J Pathol. 1992 Jun;14(1):29-33.

Abstract

The use of the colloidal-gold technique in electron microscopy immunocytochemistry has provided important information on the in situ localisation of intracellular antigens. We have developed a post-embedding technique for prolactin localisation on resin-embedded human pituitary tissue sections by the use of the protein-A gold conjugate. Human pituitary tissue obtained at autopsy was processed for electron microscopical study without post-osmication and then embedded in Epon. The indirect immunoperoxidase method was used for light microscopical targetting of lactotroph cells for subsequent electron microscopical antigen localisation. Ultra-thin sections were labelled with human anti-human prolactin followed by protein-A gold conjugate. Specific labelling was observed over secretory granules with a density of 15-30 particles per granule, as determined by the Quantimet 570 image analysis system. This technique provides a means of studying the pathophysiology of hormonal secretion at ultrastructural level and can be a useful tool in diagnostic and research investigations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Epoxy Resins
  • Humans
  • Immunohistochemistry
  • Microscopy, Electron
  • Pituitary Gland / chemistry*
  • Pituitary Gland / ultrastructure
  • Prolactin / analysis*
  • Tissue Embedding / methods*

Substances

  • Epoxy Resins
  • EPON
  • Prolactin