[A method of isolation and properties of DNA-dependent DNA-polymerase epsilon from human placenta]

Mol Biol (Mosk). 1992 Sep-Oct;26(5):999-1010.
[Article in Russian]

Abstract

DNA polymerase epsilon was purified to near homogeneity from human placenta. The enzyme has one subunit (170 kDa, sedimentation coefficient 8.2S), intrinsic 3'-5'-exonuclease activity, it is independent on PCNA and high processivity on poly(dA)-oligo(dT) template-primer without PCNA. It was shown, that the enzyme incorporates 3'-amino-2',3'-dideoxythymidine 5'-triphosphate in DNA, after that synthesis is stopped. Simultaneously DNA polymerase alpha was purified.

MeSH terms

  • Base Sequence
  • Chromatography, Liquid
  • DNA / biosynthesis
  • DNA Polymerase II / isolation & purification
  • DNA Polymerase II / metabolism
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Molecular Sequence Data
  • Placenta / enzymology*
  • Pregnancy
  • Substrate Specificity

Substances

  • DNA
  • DNA Polymerase II
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase