Abstract
DNA polymerase epsilon was purified to near homogeneity from human placenta. The enzyme has one subunit (170 kDa, sedimentation coefficient 8.2S), intrinsic 3'-5'-exonuclease activity, it is independent on PCNA and high processivity on poly(dA)-oligo(dT) template-primer without PCNA. It was shown, that the enzyme incorporates 3'-amino-2',3'-dideoxythymidine 5'-triphosphate in DNA, after that synthesis is stopped. Simultaneously DNA polymerase alpha was purified.
MeSH terms
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Base Sequence
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Chromatography, Liquid
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DNA / biosynthesis
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DNA Polymerase II / isolation & purification
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DNA Polymerase II / metabolism
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DNA Polymerase III
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DNA-Directed DNA Polymerase / isolation & purification*
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DNA-Directed DNA Polymerase / metabolism
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Electrophoresis, Polyacrylamide Gel
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Female
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Humans
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Molecular Sequence Data
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Placenta / enzymology*
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Pregnancy
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Substrate Specificity
Substances
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DNA
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DNA Polymerase II
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DNA Polymerase III
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DNA-Directed DNA Polymerase