Abstract
Many hematopoietic cells undergo apoptosis when deprived of specific cytokines. Lipocalin 24p3, reported to be induced in hematopoietic cells by interleukin 3 (IL-3) depletion, induces hematopoietic cell apoptosis despite the presence of IL-3. As granulocyte colony stimulating factor (G-CSF) depletion also induces the apoptosis of G-CSF-dependent cell line cells, we examined the effect of 24p3 on the apoptotic function induced by G-CSF depletion. 24p3 was induced by the depletion of IL-3, but not G-CSF, in cytokine-dependent cell lines. Although 24p3 suppressed growth induced by IL-3, it did not influence G-CSF-dependent cell growth. These observations show that 24p3 is not involved in the G-CSF withdrawal-induced apoptosis, although it is essential in IL-3 withdrawal-induced apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute-Phase Proteins / analysis
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Acute-Phase Proteins / genetics
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Acute-Phase Proteins / physiology*
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Animals
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Apoptosis / physiology*
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Cell Differentiation
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Cell Division / physiology
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Cell Line
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Culture Media, Conditioned
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Gene Expression
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Granulocyte Colony-Stimulating Factor / metabolism*
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Hematopoietic Stem Cells / cytology*
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Humans
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Interleukin-3 / metabolism*
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Lipocalin-2
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Lipocalins
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Mice
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Neutrophils / cytology
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Oncogene Proteins / analysis
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Oncogene Proteins / genetics
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Oncogene Proteins / physiology*
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Polymerase Chain Reaction
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Proto-Oncogene Proteins
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Recombinant Proteins
Substances
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Acute-Phase Proteins
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Culture Media, Conditioned
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Interleukin-3
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LCN2 protein, human
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Lipocalin-2
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Lipocalins
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Oncogene Proteins
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Proto-Oncogene Proteins
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Recombinant Proteins
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Lcn2 protein, mouse
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Granulocyte Colony-Stimulating Factor