Abstract
Both HIV-1 reverse transcriptase (RT) and topoisomerase I bind to structural RNAs and they cooperate to synthesize cDNA during the replication of HIV-1. In this study, we find that human topoisomerase I exclusively dissociated HIV-1 reverse transcriptase, which strongly binds to structural RNAs. Meanwhile, topoisomerase I did not dissociate either HIV-1 nucleocapsid proteins or murine leukemia virus RT which was bound to RNA. We propose that human topoisomerase I may regulate the binding of RT to RNAs and play a pivotal role in HIV-1 replication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Replication
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DNA Topoisomerases, Type I / genetics
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DNA Topoisomerases, Type I / metabolism*
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Gene Products, gag / genetics
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Gene Products, gag / metabolism
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HIV Reverse Transcriptase / genetics
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HIV Reverse Transcriptase / metabolism*
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HIV-1 / enzymology*
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HIV-1 / genetics
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HIV-1 / physiology
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Humans
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In Vitro Techniques
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Kinetics
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Leukemia Virus, Murine / enzymology
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Nucleocapsid Proteins / metabolism
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RNA / genetics
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RNA / metabolism
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RNA, Spliced Leader / genetics
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RNA, Spliced Leader / metabolism
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RNA-Directed DNA Polymerase / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Surface Plasmon Resonance
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Virus Replication
Substances
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Gene Products, gag
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Nucleocapsid Proteins
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RNA, Spliced Leader
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Recombinant Proteins
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RNA
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HIV Reverse Transcriptase
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RNA-Directed DNA Polymerase
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DNA Topoisomerases, Type I