Abstract
The molecular basis of host-tumor interaction in HLA-A31+ cancer patients has not been well understood. This lack of clarification is hampering the development of specific immunotherapies for these patients. This study aimed to identify a set of CTL-epitope peptides applicable for the specific immunotherapy of cancer patients with HLA-A31 allele. HLA-A31 allele is expressed in 5-10% of the world population, with the highest expression among Brazilian Amerinds (65%), and the lowest in the Eskimo population (0%). We report herein four cDNAs encoding CTL-epitopes and 7 epitope peptides with the ability to induce HLA-A31-restricted CTLs cytotoxic to tumor cell lines in the peripheral blood mononuclear cells of HLA-A31+ cancer patients. These peptides might be useful for the development of a peptide-based immunotherapy for HLA-A31+ cancer patients.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Antigens, Neoplasm
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Blotting, Northern
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COS Cells
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Cancer Vaccines / therapeutic use*
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Cell Line, Tumor
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DNA, Complementary / metabolism
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Dose-Response Relationship, Drug
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Epitopes / chemistry
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Epitopes, T-Lymphocyte
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HLA-A Antigens / biosynthesis*
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HLA-A Antigens / genetics
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Humans
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Immunotherapy / methods*
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Leukocytes, Mononuclear / metabolism
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Molecular Sequence Data
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Neoplasms, Glandular and Epithelial / ethnology
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Neoplasms, Glandular and Epithelial / genetics
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Neoplasms, Glandular and Epithelial / metabolism*
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Peptides / chemistry
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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T-Lymphocytes, Cytotoxic / metabolism
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Vaccines, Subunit / chemistry*
Substances
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Antigens, Neoplasm
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Cancer Vaccines
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DNA, Complementary
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Epitopes
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Epitopes, T-Lymphocyte
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HLA-A Antigens
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HLA-A31 antigen
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Peptides
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RNA, Messenger
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Vaccines, Subunit