Aim: To investigate the chemical constituents of Cyanotis arachnoidea.
Methods: By using chromatographic methods for separation and combination with spectral analysis, their chemical structures were determined.
Results: Six compounds were identified as ajugasterone C-20, 22-acetonide (1), 20-hydroxyecdysone-20, 22-acetonide (2), 22-oxo-ajugasterone C (3), 22-oxo-20-hydroxyecdysone (4), beta-sitosterol (5), daucosterol (6).
Conclusion: Compound 3 is a new compound, 4 was a new natural compound.