Abstract
A direct continuous fluorescence assay for translocase II MurG based on fluorescence resonance energy transfer (FRET) has been developed using a 6-substituted fluorescent analogue of UDP-N-acetylglucosamine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Outer Membrane Proteins / analysis*
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Catalysis
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Cell Line
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Cell Membrane / metabolism
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Escherichia coli / enzymology
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Fluorescence Resonance Energy Transfer / methods
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Fluorescent Dyes / chemistry*
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Kinetics
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Lipid Metabolism
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Lipids / chemistry
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Molecular Structure
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N-Acetylglucosaminyltransferases / analysis*
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Peptidoglycan / chemistry
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Peptidoglycan / metabolism*
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Substrate Specificity
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Uridine Diphosphate N-Acetylglucosamine / chemistry*
Substances
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Bacterial Outer Membrane Proteins
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Fluorescent Dyes
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Lipids
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Peptidoglycan
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Uridine Diphosphate N-Acetylglucosamine
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N-Acetylglucosaminyltransferases
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UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase