Abstract
[small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed [small beta]-galactosidase was determined using p-aminophenyl [small beta]-d-galactopyranoside (PAPG) as a substrate.
MeSH terms
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Aminophenols / chemistry
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Collagen
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Electrochemistry / methods*
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Galactosides / chemistry
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Galactosides / metabolism
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Gels
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Genes, Reporter*
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Microcomputers
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Quinones / chemistry
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Time Factors
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beta-Galactosidase / analysis*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Aminophenols
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Galactosides
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Gels
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Quinones
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iminoquinone
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4-aminophenyl-beta-galactoside
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Collagen
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beta-Galactosidase