Abstract
Unlike major histocompatibility proteins, which bind peptides, CD1 proteins display lipid antigens to T cells. Here, we report that CD1a presents a family of previously unknown lipopeptides from Mycobacterium tuberculosis, named didehydroxymycobactins because of their structural relation to mycobactin siderophores. T cell activation was mediated by the alphabeta T cell receptors and was specific for structure of the acyl and peptidic components of these antigens. These studies identify a means of intracellular pathogen detection and identify lipopeptides as a biochemical class of antigens for T cells, which, like conventional peptides, have a potential for marked structural diversity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigen Presentation*
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / immunology*
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Antigens, Bacterial / metabolism
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Antigens, CD1 / chemistry
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Antigens, CD1 / immunology
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Antigens, CD1 / metabolism
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Cell Line
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Chromatography, High Pressure Liquid
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Humans
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Hydrogen Bonding
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Hydrophobic and Hydrophilic Interactions
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Hydroxylation
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Lipoproteins / chemistry
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Lipoproteins / immunology*
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Lipoproteins / metabolism
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Lymphocyte Activation*
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Models, Molecular
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Mycobacterium tuberculosis / growth & development
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Mycobacterium tuberculosis / immunology*
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Oxazoles / chemistry
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Oxazoles / immunology*
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Oxazoles / metabolism
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Protein Conformation
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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T-Lymphocytes / immunology*
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Transfection
Substances
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Antigens, Bacterial
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Antigens, CD1
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CD1a antigen
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Lipoproteins
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Oxazoles
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Receptors, Antigen, T-Cell, alpha-beta
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mycobactins