Abstract
2'-Deoxy-2'-fluorocytidine (FdC) is a potent inhibitor of the hepatitis C virus RNA replicon in culture, and FdC-5'-triphosphate is an effective inhibitor of the NS5B polymerase. Dynamic profiling of cell growth in an antiviral assay showed that FdC caused cytostasis due to an S-phase arrest. These observations demonstrate that FdC treatment is affecting both a viral target and a cellular target.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antiviral Agents / pharmacology*
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Cattle
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Cell Division / drug effects
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Cell Line
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Deoxycytidine / analogs & derivatives*
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Deoxycytidine / pharmacology*
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Diarrhea Viruses, Bovine Viral / genetics
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / chemical synthesis
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Enzyme Inhibitors / pharmacology
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Hepacivirus / drug effects*
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Hepacivirus / genetics*
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Humans
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RNA, Viral / biosynthesis*
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RNA, Viral / genetics*
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Replicon / drug effects*
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Replicon / genetics*
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S Phase / drug effects
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Viral Nonstructural Proteins / antagonists & inhibitors
Substances
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Antiviral Agents
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Enzyme Inhibitors
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RNA, Viral
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Viral Nonstructural Proteins
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Deoxycytidine
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NS-5 protein, hepatitis C virus
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2'-fluoro-2'-deoxycytidine