Crystallization and preliminary X-ray analysis of human angiogenin

K R Acharya; V Subramanian; R Shapiro; J F Riordan; B L Vallee

doi:10.1016/0022-2836(92)90333-f

Crystallization and preliminary X-ray analysis of human angiogenin

J Mol Biol. 1992 Dec 20;228(4):1269-70. doi: 10.1016/0022-2836(92)90333-f.

Authors

K R Acharya¹, V Subramanian, R Shapiro, J F Riordan, B L Vallee

Affiliation

¹ Department of Biochemistry, University of Bath, Claverton Down, U.K.

PMID: 1474591
DOI: 10.1016/0022-2836(92)90333-f

Abstract

Crystals of recombinant human angiogenin have been grown from solutions containing sodium potassium tartrate and polyethylene glycol as precipitants. They belong to the space group C222(1) (a = 83.36 A, b = 120.64 A, c = 37.72 A) and contain a single molecule in the asymmetric unit. The crystals diffract to at least 2.3 A resolution and are suitable for three-dimensional X-ray structural analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Humans
Proteins / chemistry*
Proteins / genetics
Recombinant Proteins / chemistry
Ribonuclease, Pancreatic*
X-Ray Diffraction

Substances

Proteins
Recombinant Proteins
angiogenin
Ribonuclease, Pancreatic