Crystallization of YloQ, a GTPase of unknown function essential for Bacillus subtilis viability

Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):329-30. doi: 10.1107/S090744490302626X. Epub 2004 Jan 23.

Abstract

YloQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 A spacing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / chemistry
  • Bacillus subtilis / enzymology*
  • Bacillus subtilis / physiology*
  • Bacterial Proteins / physiology*
  • Cell Survival
  • Crystallography, X-Ray
  • GTP Phosphohydrolases / chemistry*
  • Guanosine Triphosphate / chemistry
  • Polyethylene Glycols / chemistry
  • Protein Conformation
  • Protein Structure, Tertiary
  • Transgenes

Substances

  • Bacterial Proteins
  • Polyethylene Glycols
  • Guanosine Triphosphate
  • Adenosine Triphosphate
  • GTP Phosphohydrolases