Structure of the saccharide-binding domain of the human natural killer cell inhibitory receptor p75/AIRM1

Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):401-3. doi: 10.1107/S0907444903028439. Epub 2004 Jan 23.

Abstract

The high-resolution crystal structure of the functional N-terminal domain from the extracellular region of the human natural killer cell inhibitory receptor p75/AIRM1 or Siglec-7 has been determined at 1.45 A resolution; it was obtained from a crystal belonging to a primitive monoclinic space group, with unit-cell parameters a = 32.65, b = 49.72, c = 39.79 A, alpha = gamma = 90, beta = 113 degrees. The structure reported here belongs to a different space group than the previously described Siglec-7 structure and was obtained using a bacterial expression system. The structure unveils the fine structural requirements adopted by a natural killer cell inhibitory receptor of the Siglec family in target-cell recognition and binding.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Carbohydrates / chemistry*
  • Cloning, Molecular
  • Crystallography, X-Ray
  • Humans
  • Killer Cells, Natural / metabolism
  • Models, Molecular
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • Receptors, Immunologic / chemistry*

Substances

  • Carbohydrates
  • Receptors, Immunologic
  • adhesion inhibitory receptor molecule 1