In this study we compared the egg size, the eggshell thickness and the number of spermatozoa trapped on the perivitelline layer of the eggs in three blue tit (Parus caeruleus) populations across a heavy metal pollution gradient. No significant differences were observed in egg characteristics among study sites. Eggs from the two most polluted sites however, had significantly less spermatozoa on the perivitelline layer than eggs from the least polluted site. Our results suggest a significant effect of pollution on blue tit sperm concentrations in the infundibulum. Determining the number of spermatozoa on the egg membranes is relatively cheap and straightforward, which renders this technique a new and useful tool in biomonitoring.