An ultra small array of electrodes for stimulating multiple inputs into a single neuron

J Neurosci Methods. 2004 Feb 15;133(1-2):109-14. doi: 10.1016/j.jneumeth.2003.10.001.

Abstract

We have developed an ultra small, translucent array of electrodes for use in the parasaggital cerebellar slice preparation. This positionable array is capable of stimulating multiple independent bundles of parallel fibers (PFs), which synapse onto a single Purkinje neuron. On a silicon substrate, a low-stress silicon nitride film was used both as a structural layer and as electrical insulation. Evaporated gold pads and interconnects were sandwiched between two such layers. A bulk anisotropic silicon etch released the individual arrays. The electrodes are supported within a 2-microm-thick cantilever of translucent silicon nitride. In one design, eight 4-microm-wide square electrodes are arranged on 8-microm-centers. Another design, half the scale of the first, was also tested. The array was mounted on a micromanipulator and can be visualized by an upright microscope. It can then be positioned in the dendritic arbor of a Purkinje neuron while not disturbing a recording pipette at the soma. Paired-pulse facilitation experiments have confirmed that the electrodes are capable of stimulating non-overlapping bundles of PFs. This device will be useful for exploring spatiotemporal synaptic integration in single neurons. Potential applications in experiments on cerebellar LTD are also discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Cerebellum / cytology*
  • Electric Stimulation / methods*
  • Electrophysiology
  • Equipment Design
  • Excitatory Postsynaptic Potentials / physiology
  • In Vitro Techniques
  • Microelectrodes*
  • Purkinje Cells / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Sensitivity and Specificity
  • Silicon

Substances

  • Silicon