Total antioxidant capacity of arginine-conjugated linoleic acid (CLA) complex

J Agric Food Chem. 2004 Feb 11;52(3):439-44. doi: 10.1021/jf030186w.

Abstract

Conjugated linoleic acids (CLA) have shown diverse biological activities, including anti-carcinogenic, anti-atherosclerotic, anti-adipogenic, and anti-diabetogenic effects. Recent reports also showed that CLA has free radical scavenging capacity, which may give health benefits to human beings. However, the application of CLA as a bioactive ingredient has been limited due to its insolubility in water. To overcome this problem, we investigated antioxidant activities of arginine (Arg)-CLA, a water-soluble CLA salt, using both 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. CLA, Arg, and Arg-CLA all exerted radical scavenging activities in a dose-dependent manner in both assays. Arg-CLA at 20 mM scavenged 89 and 55% of ABTS and DPPH radicals in 3 h, respectively, whereas CLA alone quenched only 48 and 26% of them under the same conditions. The antioxidant activity of the Arg-CLA complex was found to be synergistic in ABTS assay and comparable to that of vitamin E in DPPH assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Arginine / analogs & derivatives*
  • Arginine / pharmacology
  • Benzothiazoles
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Linoleic Acids, Conjugated / chemistry
  • Linoleic Acids, Conjugated / pharmacology*
  • Picrates
  • Solubility
  • Sulfonic Acids
  • Water

Substances

  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Linoleic Acids, Conjugated
  • Picrates
  • Sulfonic Acids
  • Water
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Arginine
  • 1,1-diphenyl-2-picrylhydrazyl