An investigation of the mechanisms of electronic sensing of protein adsorption on carbon nanotube devices

J Am Chem Soc. 2004 Feb 11;126(5):1563-8. doi: 10.1021/ja038702m.

Abstract

It has been reported that protein adsorption on single-walled carbon nanotube field effect transistors (FETs) leads to appreciable changes in the electrical conductance of the devices, a phenomenon that can be exploited for label-free detection of biomolecules with a high potential for miniaturization. This work presents an elucidation of the electronic biosensing mechanisms with a newly developed microarray of nanotube "micromat" sensors. Chemical functionalization schemes are devised to block selected components of the devices from protein adsorption, self-assembled monolayers (SAMs) of methoxy(poly(ethylene glycol))thiol (mPEG-SH) on the metal electrodes (Au, Pd) and PEG-containing surfactants on the nanotubes. Extensive characterization reveals that electronic effects occurring at the metal-nanotube contacts due to protein adsorption constitute a more significant contribution to the electronic biosensing signal than adsorption solely along the exposed lengths of the nanotubes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adsorption
  • Animals
  • Biosensing Techniques / methods*
  • Cattle
  • Gold / chemistry
  • Humans
  • Microscopy, Atomic Force
  • Nanotubes, Carbon / chemistry*
  • Palladium / chemistry
  • Polyethylene Glycols / chemistry
  • Proteins / chemistry*
  • Sulfhydryl Compounds / chemistry

Substances

  • Nanotubes, Carbon
  • Proteins
  • Sulfhydryl Compounds
  • Polyethylene Glycols
  • Palladium
  • Gold