Abstract
We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alternative Splicing / physiology*
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Animals
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Cells, Cultured
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Exons
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Green Fluorescent Proteins
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Humans
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Introns
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Kidney / metabolism*
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Luminescent Proteins*
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Male
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Microscopy, Fluorescence / methods*
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Prostatic Neoplasms / genetics*
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Prostatic Neoplasms / metabolism
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RNA Precursors / genetics
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Rats
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Receptor Protein-Tyrosine Kinases / genetics*
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Receptor Protein-Tyrosine Kinases / metabolism
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Receptor, Fibroblast Growth Factor, Type 2
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Receptors, Fibroblast Growth Factor / genetics*
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Receptors, Fibroblast Growth Factor / metabolism
Substances
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Luminescent Proteins
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RNA Precursors
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Receptors, Fibroblast Growth Factor
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Green Fluorescent Proteins
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FGFR2 protein, human
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Fgfr2 protein, rat
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Receptor Protein-Tyrosine Kinases
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Receptor, Fibroblast Growth Factor, Type 2