Abstract
Results from nuclear run-off assays show that exposure of hepatocytes and Reuber H35B hepatoma cells to the tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), leads to enhanced transcription of c-Ki-ras gene. This increase in transcription in turn results in an accumulation of the functionally active c-Ki-ras message. The half life of c-Ki-ras message in both normal and transformed livers cells is not altered by TPA and is determined to be 3.5 hr. The induction of c-Ki-ras message is accompanied by an increase in the level of c-Ki-ras protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carcinogens / pharmacology*
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Cell Line, Transformed
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Cell Nucleus / drug effects
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Genes, ras / genetics*
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Half-Life
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Liver Neoplasms, Experimental / genetics*
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Protein Biosynthesis / drug effects
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Proto-Oncogene Proteins / genetics
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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Rats
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Rats, Sprague-Dawley
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Reference Values
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Tetradecanoylphorbol Acetate / pharmacology*
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Transcription, Genetic / drug effects*
Substances
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Carcinogens
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Proto-Oncogene Proteins
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RNA, Messenger
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Tetradecanoylphorbol Acetate