Abstract
Human papillomavirus (HPV) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. Of the sexually transmitted types, HPV type 16 (HPV-16) is the most commonly associated with carcinoma of the uterine cervix. Expression of the E6/E7 open reading frame of the viral genome is considered critical in the development of neoplasia. Using the CaSki cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the transcripts expressed from this region. It was found that 97.1% of the total spliced transcript is E6*I, which putatively encodes the E7 oncoprotein, while E6*II comprises 2.9% of spliced product. The ratio of E6*I to E6*II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Carcinoma / microbiology*
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Carcinoma / pathology
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DNA Probes, HPV
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Female
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Gene Expression Regulation, Neoplastic
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Gene Expression Regulation, Viral
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Humans
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Molecular Sequence Data
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Oncogene Proteins, Viral / biosynthesis
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Oncogene Proteins, Viral / genetics*
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Papillomavirus E7 Proteins
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Polymerase Chain Reaction*
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RNA Splicing
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RNA, Neoplasm / analysis*
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RNA, Viral / analysis*
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Repressor Proteins*
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Transcription, Genetic*
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Tumor Cells, Cultured / metabolism
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Tumor Cells, Cultured / microbiology*
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Uterine Cervical Neoplasms / microbiology*
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Uterine Cervical Neoplasms / pathology
Substances
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DNA Probes, HPV
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E6 protein, Human papillomavirus type 16
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Oncogene Proteins, Viral
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Papillomavirus E7 Proteins
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RNA, Neoplasm
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RNA, Viral
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Repressor Proteins
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oncogene protein E7, Human papillomavirus type 16