Abstract
We have analyzed the genomic structure of the human WT1 gene, one of the recessive oncogenes for Wilms' tumor at chromosome 11p13. By analyses of three cosmids covering the WT1 gene as well as products generated by polymerase chain reaction, cleavage sites for 10 restriction enzymes were mapped in a region of about 80 kb, and the positions of 10 exons were defined. We also mapped two polymorphic sites for TaqI. Our genomic map will be useful to analyze DNA abnormalities sometimes found in the tumors, as well as loss of heterozygosity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cosmids / genetics
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / genetics*
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Deoxyribonucleases, Type II Site-Specific
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Exons / genetics
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Genes, Wilms Tumor / genetics*
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Genome, Human
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Humans
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Introns / genetics
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Molecular Sequence Data
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Polymorphism, Genetic / genetics
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Restriction Mapping*
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WT1 Proteins
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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WT1 Proteins
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Deoxyribonucleases, Type II Site-Specific
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TCGA-specific type II deoxyribonucleases