Abstract
New monoclonal antibodies (mAbs) against HLA class I antigens were prepared and characterized. Three mAbs (CLI-1, CLI-2, CLI-3) were directed to the heavy chain of HLA class I molecules and one mAb (B2M-1) is specific to beta 2-microglobulin. Using these mAbs, it was confirmed that acid treatment selectively eliminated cell surface HLA class I antigenicity. The treatment with citric acid solution resulted in preparation of cells with lower residual HLA class I surface molecules than was seen after the treatment with glycine buffer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / chemistry*
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Antibody Specificity
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Antigens, Surface / drug effects*
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Antigens, Surface / immunology*
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Citrates*
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Citric Acid
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Fluorescent Antibody Technique
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Histocompatibility Antigens Class I / drug effects*
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Histocompatibility Antigens Class I / immunology*
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Humans
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Hydrochloric Acid*
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Hydrogen-Ion Concentration
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Mice
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Mice, Inbred BALB C
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Precipitin Tests
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Solutions
Substances
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Antibodies, Monoclonal
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Antigens, Surface
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Citrates
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Histocompatibility Antigens Class I
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Solutions
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Citric Acid
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Hydrochloric Acid