Analysis of maternal and neonatal factors that influence the nucleated and CD34+ cell yield for cord blood banking

Transfusion. 2004 Feb;44(2):262-7. doi: 10.1111/j.1537-2995.2004.00645.x.

Abstract

Background: It would be beneficial to be able to predict the cord blood (CB) cell yield from volunteer donors before cell processing.

Study design and methods: The maternal and neonatal factors that influence the total nucleated cell (TNC), CD34+ cell, and CFU-GM yields in CB collected for the Chugoku-Shikoku Cord Blood Bank were evaluated.

Results: In a univariate analysis, the volume of CB collected was significantly correlated with the TNC, CD34+ cell, and CFU-GM yields (p < 0.001). A longer cord (p < 0.001), larger placenta (p < 0.001), and bigger baby (p < 0.001) were associated with a greater volume of CB. A female baby (p < 0.05) and longer gestational age (p < 0.005) were associated with a higher TNC concentration. A younger maternal age (p < 0.05), larger birth weight (p < 0.001), shorter gestational age (p < 0.001), and shorter time from collection to processing (p < 0.05) were associated with a higher CD34+ cell concentration. A multivariate linear regression analysis was performed to predict the yield and determine first-level selection criteria to start processing when the volume of CB units was on the borderline. However, this formula might not be suitable for actual use.

Conclusion: Maternal and neonatal factors appeared to affect CB cell yields. These findings might be useful for efficiently collecting more qualified CB units.

MeSH terms

  • Age Factors
  • Antigens, CD34 / analysis
  • Blood Banking / methods*
  • Cell Nucleus
  • Female
  • Fetal Blood / cytology*
  • Gestational Age
  • Hematopoietic Stem Cells / chemistry
  • Hematopoietic Stem Cells / cytology*
  • Humans
  • Male
  • Maternal Age
  • Placenta
  • Predictive Value of Tests
  • Pregnancy
  • Regression Analysis
  • Sex Factors

Substances

  • Antigens, CD34