The trunk neural crest and its early glial derivatives: a study of survival responses, developmental schedules and autocrine mechanisms

Mol Cell Neurosci. 2004 Jan;25(1):30-41. doi: 10.1016/j.mcn.2003.09.006.

Abstract

Regulation of survival during gliogenesis from the trunk neural crest is poorly understood. Using adapted survival assays, we directly compared crest cells and the crest-derived precursor populations that generate satellite cells and Schwann cells. A range of factors that supports Schwann cells and glial precursors does not rescue crest, with the major exception of neuregulin-1 that rescues crest cells provided they contact the extracellular matrix. Autocrine survival appears earlier in developing satellite cells than Schwann cells. Satellite cells also show early expression of S100beta, BFABP and fibronectin and early survival responses to IGF-1, NT-3 and PDGF-BB that in developing Schwann cells are not seen until the precursor/Schwann cell transition. These experiments define novel differences between crest cells and early glia and show that entry to the glial lineage is an important point for regulation of survival responses. They show that survival mechanisms among PNS glia differ early in development and that satellite cell development runs ahead of schedule compared to Schwann cells in several significant features.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autocrine Communication / physiology*
  • Cell Differentiation / physiology*
  • Cell Line, Tumor
  • Cell Lineage / physiology
  • Cell Survival / physiology
  • Cells, Cultured
  • Fetus
  • Fibronectins / metabolism
  • Nerve Growth Factors / metabolism
  • Nerve Growth Factors / pharmacology
  • Neural Crest / cytology
  • Neural Crest / embryology*
  • Neural Crest / metabolism
  • Neuregulin-1 / metabolism
  • Neuregulin-1 / pharmacology
  • Neuroglia / cytology
  • Neuroglia / metabolism*
  • Organ Culture Techniques
  • Peripheral Nervous System / cytology
  • Peripheral Nervous System / embryology*
  • Peripheral Nervous System / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • S100 Proteins / metabolism
  • Satellite Cells, Perineuronal / cytology
  • Satellite Cells, Perineuronal / metabolism
  • Schwann Cells / cytology
  • Schwann Cells / metabolism
  • Stem Cells / cytology
  • Stem Cells / metabolism*

Substances

  • Fibronectins
  • Nerve Growth Factors
  • Neuregulin-1
  • S100 Proteins
  • S100A1 protein