Abstract
Peptide antagonists of the human papillomavirus type 11 (HPV-11) E2-DNA association were identified using a filamentous bacteriophage random peptide library. Synthetic peptides antagonized the E2-DNA interaction, effectively blocked E2-mediated transcriptional activation of a reporter gene in cell culture, and inhibited E1-E2-mediated HPV-11 DNA replication in vitro. These peptides may prove to be useful tools for characterizing E2 function and for exploring the effectiveness of E2-inhibitor-based treatments for HPV-associated diseases.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antiviral Agents / chemistry
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Antiviral Agents / pharmacology
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Cell-Free System
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Chlorocebus aethiops
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DNA Replication / drug effects*
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DNA, Viral / antagonists & inhibitors
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DNA, Viral / metabolism*
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Electron Spin Resonance Spectroscopy
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Gene Expression Regulation, Viral / drug effects*
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Genes, Reporter / genetics
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Inhibitory Concentration 50
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Molecular Sequence Data
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Papillomaviridae / drug effects
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Papillomaviridae / genetics
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Papillomaviridae / physiology
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Peptide Library
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / pharmacology*
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Protein Binding / drug effects
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Thermodynamics
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Trans-Activators / antagonists & inhibitors
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Transcriptional Activation / drug effects*
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Vero Cells
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Viral Proteins / antagonists & inhibitors*
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Viral Proteins / genetics
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Viral Proteins / metabolism
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Virus Replication / drug effects*
Substances
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Antiviral Agents
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DNA, Viral
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E2 protein, Human papillomavirus type 11
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Peptide Library
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Peptides
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Trans-Activators
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Viral Proteins