Abstract
Jaagsiekte sheep retrovirus (JSRV) infects lung epithelial cells in sheep, and oncoretroviral vectors bearing JSRV Env can mediate transduction of human cells, suggesting that such vectors might be useful for lung-directed gene therapy. Here we show that JSRV Env can also efficiently pseudotype a human immunodeficiency virus type 1-based lentiviral vector, a more suitable vector for transduction of slowly dividing lung epithelial cells. We created several chimeric Env proteins that, unlike the parental Env, do not transform rodent fibroblasts but are still capable of pseudotyping lentiviral and oncoretroviral vectors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Adhesion Molecules / metabolism
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Cell Division
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Cells, Cultured
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Epithelial Cells / cytology
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Epithelial Cells / virology
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Fibroblasts
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GPI-Linked Proteins
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Gene Products, env / chemistry
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Gene Products, env / genetics
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Gene Products, env / metabolism*
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Genetic Therapy / methods*
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Genetic Vectors / genetics*
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Genetic Vectors / physiology*
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HIV-1 / genetics*
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HIV-1 / physiology*
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Humans
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Hyaluronoglucosaminidase / metabolism
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Jaagsiekte sheep retrovirus / genetics
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Jaagsiekte sheep retrovirus / metabolism*
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Lung / cytology
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Lung / virology
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Molecular Sequence Data
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Moloney murine leukemia virus / genetics
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Moloney murine leukemia virus / physiology
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Organ Specificity
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Plasmids / genetics
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Receptors, Virus / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Rodentia
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Transduction, Genetic
Substances
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Cell Adhesion Molecules
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GPI-Linked Proteins
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Gene Products, env
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Receptors, Virus
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Recombinant Fusion Proteins
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Hyal2 protein, human
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Hyaluronoglucosaminidase