Thyroid cell proliferation in Graves' disease. Use of MIB-1 monoclonal antibody

Acta Cytol. 2004 Jan-Feb;48(1):57-63. doi: 10.1159/000326284.

Abstract

Objective: To measure thyroid cell proliferation in patients with Graves' disease (GD) before and during treatment with antithyroid drugs.

Study design: Patients were assessed by fine needle aspiration biopsy before (n = 20) and after 4 (n = 19) and 12 months of treatment (n = 15) with propylthiouracil or methimazole. Cell proliferation index (CPI) was estimated by immunocytochemistry using MIB-1. CPI was studied in relation to the cytologic parameters of the smears; clinical parameters, such as Wayne's Clinical Index (WCI) and time without treatment; laboratory parameters, such as 131I uptake and dosage of serum free thyroxin and thyroid-stimulating hormone; and thyroid ultrasound.

Results: CPI varied from 0.00% to 25.00% before treatment, 0.00% to 23.00% at 4 months and 0.00% to 14.84% at 12 months. CPI median values were 6.50%, 4.30% and 3.30%, respectively (before and after 4 months and 12 months of treatment). CPI had a positive correlation with WCI and FT4 at 12 months of treatment.

Conclusion: Thyroid CPI in GD varies from case to case. However, due to its decreasing pattern during follow-up and its positive correlation with thyrotoxicosis severity, CPI may indicate the functional status of the gland and contribute to a better understanding of GD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Antinuclear* / metabolism
  • Antibodies, Monoclonal* / metabolism
  • Antithyroid Agents / pharmacology
  • Biopsy, Fine-Needle
  • Cell Division / drug effects
  • Cell Division / physiology
  • Child
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology*
  • Female
  • Graves Disease / drug therapy
  • Graves Disease / pathology*
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen / immunology
  • Ki-67 Antigen / metabolism
  • Male
  • Methimazole / pharmacology
  • Middle Aged
  • Predictive Value of Tests
  • Propylthiouracil / pharmacology
  • Reproducibility of Results
  • Thyroid Gland / drug effects
  • Thyroid Gland / metabolism
  • Thyroid Gland / pathology*

Substances

  • Antibodies, Antinuclear
  • Antibodies, Monoclonal
  • Antithyroid Agents
  • Ki-67 Antigen
  • MIB-1 antibody
  • Methimazole
  • Propylthiouracil