Identification of murine T-cell epitopes in Ebola virus nucleoprotein

Virology. 2004 Jan 5;318(1):224-30. doi: 10.1016/j.virol.2003.09.016.

Abstract

CD8 T cells play an important role in controlling Ebola infection and in mediating vaccine-induced protective immunity, yet little is known about antigenic targets in Ebola that are recognized by CD8 T cells. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mice immunized with vectors encoding Ebola nucleoprotein (NP). CD8 T-cell responses were mapped to a H-2(d)-restricted epitope (NP279-288) and two H-2(b)-restricted epitopes (NP44-52 and NP288-296). The identification of these epitopes will facilitate studies of immune correlates of protection and the evaluation of vaccine strategies in murine models of Ebola infection.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CD8-Positive T-Lymphocytes / immunology
  • Disease Models, Animal
  • Ebolavirus / immunology*
  • Epitope Mapping*
  • Epitopes, T-Lymphocyte / immunology*
  • Hemorrhagic Fever, Ebola / immunology
  • Hemorrhagic Fever, Ebola / prevention & control
  • Histocompatibility Antigens Class I / metabolism
  • Humans
  • Immunization
  • Immunization, Secondary
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nucleocapsid Proteins / chemistry
  • Nucleocapsid Proteins / immunology*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*

Substances

  • Epitopes, T-Lymphocyte
  • Histocompatibility Antigens Class I
  • Nucleocapsid Proteins
  • Viral Vaccines
  • nucleocapsid protein, Ebola virus