Abstract
CD8 T cells play an important role in controlling Ebola infection and in mediating vaccine-induced protective immunity, yet little is known about antigenic targets in Ebola that are recognized by CD8 T cells. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mice immunized with vectors encoding Ebola nucleoprotein (NP). CD8 T-cell responses were mapped to a H-2(d)-restricted epitope (NP279-288) and two H-2(b)-restricted epitopes (NP44-52 and NP288-296). The identification of these epitopes will facilitate studies of immune correlates of protection and the evaluation of vaccine strategies in murine models of Ebola infection.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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CD8-Positive T-Lymphocytes / immunology
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Disease Models, Animal
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Ebolavirus / immunology*
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Epitope Mapping*
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Epitopes, T-Lymphocyte / immunology*
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Hemorrhagic Fever, Ebola / immunology
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Hemorrhagic Fever, Ebola / prevention & control
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Histocompatibility Antigens Class I / metabolism
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Humans
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Immunization
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Immunization, Secondary
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Molecular Sequence Data
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Nucleocapsid Proteins / chemistry
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Nucleocapsid Proteins / immunology*
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Viral Vaccines / administration & dosage
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Viral Vaccines / immunology*
Substances
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Epitopes, T-Lymphocyte
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Histocompatibility Antigens Class I
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Nucleocapsid Proteins
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Viral Vaccines
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nucleocapsid protein, Ebola virus