An arginine-histone methyltransferase, CARMER, coordinates ecdysone-mediated apoptosis in Drosophila cells

J Biol Chem. 2004 Apr 30;279(18):18467-71. doi: 10.1074/jbc.M400972200. Epub 2004 Feb 19.

Abstract

Developmentally programmed cell death is regulated by a balance between pro- and anti-death signaling. During Drosophila metamorphosis, the removal of larval tissues is dependent on the steroid hormone ecdysone, which controls the levels of pro- and anti-death molecules. Ecdysone binds to its heterodimeric receptor ecdysone receptor/ultraspiracle to mediate transcription of primary response genes. Here we show that CARMER, an arginine-histone methyltransferase, is critical in coordinating ecdysone-induced expression of Drosophila cell death genes. Ablation of CARMER blocks ecdysone-induced cell death in Drosophila cells, but not apoptosis induced by cell stress. We demonstrate that CARMER associates with the ecdysone receptor complex and modulates the ecdysone-induced transcription of a number of apoptotic genes. Thus, the chromatin-modifying protein, CARMER, modulates cell death by controlling the hormone-dependent expression of the core cell death machinery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis / genetics*
  • Drosophila / cytology*
  • Ecdysone / physiology*
  • Gene Expression Regulation, Developmental
  • Protein-Arginine N-Methyltransferases / metabolism
  • Protein-Arginine N-Methyltransferases / physiology*
  • RNA, Messenger / analysis
  • Receptors, Steroid / metabolism
  • Sequence Alignment
  • Transcription, Genetic

Substances

  • RNA, Messenger
  • Receptors, Steroid
  • ecdysone receptor
  • Ecdysone
  • Protein-Arginine N-Methyltransferases