Abstract
In this study, reverse transcriptase (RT)- and integrase (IN)-defective human immunodeficiency virus type 1 (HIV-1) was transcomplemented with Vpr-RT-IN fusion proteins to delineate pol sequences important for HIV-1 replication. Our results reveal that a 194-bp sequence encompassing the 3'end of the IN gene and containing the central DNA flap is necessary and sufficient for efficient HIV-1 single-cycle replication in dividing and nondividing cells. Furthermore, we show that the central DNA flap enhances HIV-1 single-round replication by five- to sevenfold, primarily by facilitating nuclear import of proviral DNA. In agreement with previous reports, our data support a functional role of the central DNA flap during the early stages of HIV-1 infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Nucleus / virology
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DNA, Viral / biosynthesis*
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DNA, Viral / chemistry
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Gene Products, pol / chemistry
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Gene Products, pol / genetics
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Gene Products, vpr / genetics
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Genetic Complementation Test
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HIV Integrase / genetics
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HIV Integrase / metabolism
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HIV Reverse Transcriptase / genetics
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HIV Reverse Transcriptase / metabolism
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HIV-1 / genetics
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HIV-1 / pathogenicity*
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HIV-1 / physiology
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Humans
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Leukocytes, Mononuclear / virology
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Recombinant Fusion Proteins
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Virus Integration
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Virus Replication
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vpr Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Viral
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Gene Products, pol
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Gene Products, vpr
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Recombinant Fusion Proteins
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vpr Gene Products, Human Immunodeficiency Virus
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HIV Integrase
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HIV Reverse Transcriptase